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yqeL [2019-07-05 11:32:53]
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yqeL [2019-07-05 11:32:53]

ribosomal silencing factor
Locus
BSU25620
Isoelectric point
4.37
Molecular weight
13.16 kDa
Protein length
118 aa Sequence Blast
Gene length
357 bp Sequence Blast
Function
translation
Product
ribosomal silencing factor
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other

    • Gene

    Coordinates
    2,642,841 → 2,643,197

    The protein

    Catalyzed reaction/ biological activity

  • silences translation by binding to the L14 protein of the large ribosomal subunit and, as a consequence, impairs subunit joining PubMed

    • Protein family

  • Iojap family (according to Swiss-Prot)

    • Structure

  • 2O5A (from B. halodurans, 65% identity)

    • Interactions

  • RplN-RsfS PubMed, the interaction serves to silence translation PubMed

    • Additional information

  • the protein is enriched in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed

    • Expression and Regulation

    Operons

    Genes
    yqeG-yqeH-aroD-yqeI-nadD-yqeK-rsfS-yqeM
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-C500 (yqeL::erm), available at the NBRP B. subtilis, Japan
  • BKE25620 (ΔrsfS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATTCACAAATTCCTCC, downstream forward: _UP4_GATCTTGACTTTGGAATGAA
  • BKK25620 (ΔrsfS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATTCACAAATTCCTCC, downstream forward: _UP4_GATCTTGACTTTGGAATGAA

    • References

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